P1431
 |     |  |
 | Calmodulin exists as a long dumbbell shaped molecule. Each globular end contains two calcium binding domains. Upon binding calcium, calmodulin undergoes a conformational change in which the hydrophobic regions become exposed. These hydrophobic regions are believed to be involved with enzyme binding. |
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Useful Links & Tools
- MSDS
- Certificate of Analysis
- Certificate of Origin
- P1431 - Datasheet (86 KB)
- P1431 - Enzyme Assay (24 KB)
- Transient Receptor Potential Channels (216 KB )
- InsP3/Ryanodine Receptors (253 KB )
P1431
Sigma
Calmodulin from bovine testes
BioUltra, ≥98% (SDS-PAGE), lyophilized powder, ≥40,000 units/mg protein (Lowry)
| Synonym: | CaM, Phosphodiesterase 3′:5′-cyclic nucleotide activator |
| CAS Number: | 77107-46-1 |
| MDL number: | MFCD00131869 |
Description
| Biochem/physiol Actions | Ca2+ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca2+ homeostasis. |
| Unit Definition | One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca2+. |
Properties
| grade | BioUltra |
| assay | ≥98% (SDS-PAGE) |
| form | lyophilized powder |
| total impurities | salt, essentially free |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Watterson, D.M., The complete amino acid sequence of the Ca2+-dependent modulator protein (calmodulin) of bovine brain. J. Biol. Chem. 255, 962, (1980) |
| Beavo, J.A., Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms. Physiol. Rev. 75, 725-748, (1995) | |
| Cheung, W.Y., Calmodulin plays a pivotal role in cellular regulation. Science 207, 19-27, (1980) | |
| Bredt, D., and Snyder, S.H., Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme. Proc. Natl. Acad. Sci. U. S. A. 87, 682, (1990) | |
| Klee, C.B., Isolation and characterization of bovine brain calcineurin: a calmodulin-stimulated protein phosphatase. Meth. Enzymol. 102, 227, (1983) | |
| Carafoli, E., Biogenesis: plasma membrane calcium ATPase: 15 years of work on the purified enzyme. FASEB J. 8, 993, (1994) | |
| Stull, J.T., Ca2+/calmodulin-dependent myosin light-chain kinases. Adv. Second Messenger Phosphoprotein Res. 31, 141, (1997) | |
| Nairn, A.C., and Picciotto, M.R., Calcium/calmodulin-dependent protein kinases. Semin. Cancer Biol. 5, 295, (1994) | |
| Lee, H.C., Sensitization of calcium-induced calcium release by cyclic ADP-ribose and calmodulin. J. Biol. Chem. 270, 9060, (1995) |
